Shoot Proliferation of Populus euramericana(Populus deltoides X P. nigra) through in vitro Tissue Culture

• 발행기관: 한국자원식물학회
• 저자명: Kang, Ho-Duck Moon, Heung-Kyu
• 간행물 정보: 『Plant Resources』Vol.4 No.3, 111~120쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2001.12.31

영문 초록

The efficiency of in vitro regeneration of four clones of Populus euramericana, Canada blanc, Eugenii, I-45/51, and Wisconsin #5, was examined. Cytokinins and the combinations with auxins affected the rate of regeneration from the explants of root segments, stem internodes, and leaf discs. Overall, BA and the combination with auxins were effective in root segments and leaf discs of the Canada blanc clone, whereas zeatin and the combination with auxins were important in stem internodes of the Wisconsin #5 clone. The highest number of shoots averaging 17.6 ± 0.47 from root segments in the Canada blanc clone,18.2 ± 3.0 from stem internodes in the Wisconsin #5 clone, and 17.8 ± 1.92 from leaf discs in the Canada blanc clone were obtained with 2.0 mg/1 BA, 2.0 mg/l zeatin combined with 0.2 mg/l IAA, and 0.5 mg/l BA combined with 0.05 mg/l 2,4-D, respectively. In particular, the addition of 2,4-D into cytokinin medium promoted shoot proliferation.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

LITERATURE CITED

키워드

Populus euramericana in vitro clonal proliferation root stem and leaf culture