Lilium longiflorum x L. X elegans의 자방배양에 의해 얻어진 잡종 F1의 핵형 , 감수분열 및 lsozyme에 대한 연구

• 영문명: Study of Karyotype, Meiosis and Isozyme of Hybrid from cross Lilium longiflorum x L. X elegans
• 발행기관: 한국자원식물학회
• 저자명: 윤의수(Eui Soo Yoon) 이상래(Sang Rae Lee)
• 간행물 정보: 『한국자원식물학회지』제1권 제1호, 80~87쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1988.10.30

영문 초록

Hybries which was made up by chromosome of L. longiflorum and L. x elegans, using root-tip individual which was obtained through ovary slice culture, and root-tip of these parents, with hoirugen staining, gimsa staining and Q-H staining inaccordance with the location and the existence of secondary construction which waslocating near short arm centromere of No, 1,2,6,9. In metaphase of meiosis ofhybrid which was made up by univalent from 2 individuals to 10 individuals wasobserved, and nuclear plate which was having abnormal type s synthesis amounted to91% of all cells whieh were observed. This result showed the fact that someobstacle arose annormal progress of the divission after that time. 63% of the cellshad micronucleus from 1 individlial to 4 individuals in tetrad phase of meiosisdivision. The peroxidase and α -estelase zymogram phenotypes of parents andhybrids were determined using agarlose IEF gel. Crosses were performed betweenparents bearing dissimilar allelomorphs in orther to discern the genetic control ofthe resolved enzymes. Genetic variation of hybrids were detected at all but 2 plant progenies.