Effect of Plant Growth Regulators on Clonal Production through Basal Stem Explant Cultures of a Phalaenopsis Hybrid

• 발행기관: 한국자원식물학회
• 저자명: Jo, Man-Hyun Ham, In-ki Park, Sang-kyu Seo, Gwan-Seok Han, Gyu-Heung Woo, In-Shik
• 간행물 정보: 『Plant Resources』Vol.6 No.2, 89~93쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2003.08.31

영문 초록

This study was conducted to develop the clonal propagation technique through in vitro culture using basal stem explants in Phalaenopsis hybrid grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when basal stem explants were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 ml/L coconut water, 1 mg/L NAA, 5 mg/L 2iP and 2.5g/L gel rite. PLBs transferred to Hyponex medium were regenerated to plantlets. Plantlets transferred to plastic pots containing spagnum moss were developed and successfully acclimatized under greenhouse. The flower was bloomingly opened in plants regenerated from basal stem explants. The flower was not different from both mother plant and plant induced through clonal propagation of Phalaenopsis hybrid.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

LITERATURE CITED

키워드

flowering PLB regeneration VW medium