Molecular Characterization and Expression of CuZn-superoxide Dismutase (PSOD1) from Populus alba×Populus glandulosa

• 발행기관: 한국자원식물학회
• 저자명: Lee Jun-Won In Jun-Gyo Lee Bum-Soo Choi Yong-Eui Kim Jin-Ju Yang Deok-Chun
• 간행물 정보: 『Plant Resources』Vol.8 No.1, 52~59쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2005.04.30

영문 초록

A cDNA, PSOD1, encoding cytosolic copper/zinc superoxide dismutase (CuZn-SOD) was cloned and characterized from a full length cDNA library prepared from Populus alba×Populus glandulosa cultured in vitro. A PSOD1, is 725 nucleotides long and has an open reading frame of 459 bp with 152 amino acid residues (pI 5.43). The deduced amino acid sequence of PSOD1 perfect matched to the previously reported CuZn-SOD (CAC33845.1). Consensus amino acid residues (His-45, -47, -62, -70, -79, -119) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of PSOD1 exhibited the high level of similarity from 100 to 85% among previously registered SOD genes. The expression of PSOD1 in poplar increased at the 1 mM H₂O₂ and drought stress during 30 min and 60 min, but the ozone treated poplar increased at 30 min in the early time and then decreased at 60 min.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

LITERATURE CITED

키워드

Abiotic stress Populus alba×Populus glandulosa RT-PCR superoxide dismutase