Enhancement of In Vitro Regeneration of Several Ocimum Species and Varieties

• 발행기관: 한국자원식물학회
• 저자명: Park Chung-Heon Phippen Winthrop B. Simon James E. NamKoong Seung-Bak Seong Nak-Sul
• 간행물 정보: 『Plant Resources』Vol.8 No.3, 275~280쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2005.12.31

영문 초록

Tissue culture systems to optimize regeneration plant species of Ocimum spp were evaluated as a method to micropropagate individual plants and to better study their biology in vitro. Ocimum species were also evaluated for the production of natural plant products during and following the regeneration process. The primary goal of this project was to enhance the regeneration efficiency of basil. Several factors were examined using different Ocimum species and commercial varieties. The effect of cytokinin combination, activated charcoal, gelling agents, and different carbon sources were investigated. Anthocyanin callus spots were produced only in four varieties among six tested. Sweet Dani showed the best results on anthocyanin accumulation, while African beauty , Tree basil and Methylcinnamate produced only a few spots. Shoot regeneration was only achieved from Sweet Dani explants. As the activated charcoal concentration increased, callus formation rate decreased respectively compare to the controls for all varieties. There was a decrease in callus growth with increasing concentration of agar and phytagel.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

LITERATURE CITED

키워드

basil regeneration tissue culture