학술논문
An Efficient Selection Scheme for Agrobacterium-mediated Co-transformation of Rice Using Two Selectable Marker Genes hpt and bar
이용수 2
- 영문명
- 발행기관
- 한국육종학회
- 저자명
- Soo In Sohn Yul Ho Kim Joon Hyeong Cho Ju Kon Kim Jang Yong Lee
- 간행물 정보
- 『한국육종학회지』Vol.38 No.3, 173~179쪽, 전체 7쪽
- 주제분류
- 농수해양 > 기타농수해양
- 파일형태
- 발행일자
- 2006.06.30
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국문 초록
영문 초록
A stable, efficient, and reliable selection system using bar and hpt as selectable markers for Agrobacterium mediated co-transformation of rice with two separate vectors was developed. Two plasmids, pMJC-GB-IFS2 and pMJC-GH-CHR carrying bar or hpt gene as selectable markers and isoflavone synthase 2 (IFS2) gene or chalcone reductase (CHR) gene as target genes, respectively, were used for co-transformation. Between the two selection schemes, either the selection first for hygromycin B resistance during the callus proliferation stage and later for phosphinothricine (PPT) resistance at the shoot regeneration stage or vice versa, the former selection scheme was far more effective obtaining co-transformed transgenic plants (100%) than the latter (12.8%), meaning that the PPT selection during the callus proliferation stage resulted in producing many escapes without transformed bar gene. Therefore, it was suggested that the hygromycin B selection must precede the PPT selection for efficient Agrobacterium mediated co-transformation of rice.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
LITERATURES CITED
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