動物 免疫 抑制劑를 利用한 glycine屬의 種間交雜

• 영문명: Interspecific hybridization of glycine species using animal immunosuppressants
• 발행기관: 한국육종학회
• 저자명: Young Hyun Hwang(黃永鉉) Chong Suk Huh(許彰錫)
• 간행물 정보: 『한국육종학회지』Vol.25 No.4, 278~285쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1993.12.30

영문 초록

This study was carried out to investigate the possibility of the use of immunosuppressive agents(ISA) which are commonly used for the elimination of immune system during the organ or tissue transfer in the fields of human medical science for the elimination of post-fertilization barrier which is one of the main obstacles in the inter-subgeneric or inter-specific hybridization of soybeans. Some of the results obtained are summerized as follows ; Even with dried seeds in pod shell, the successful seed germination into complete plants by the conventional filter paper method was possible for the seeds of 25 days after flowering for regular seeds. But, in case of the hybrid seeds of G. max×G. tomentella, the seed development was completely ceased within 20 days after crossing. Considering the development of G. max seeds, it was inferred that the development of hybrid seeds was ceased much prior to they have normal germinability. Some varietal difference in the crossability of G. max with G. tomentella was recognized but high degree of crossability of G. max with four-types of chromosome number in G. tomentella, of which diploid chromosome number are 38, 40, 78 and 80, was possible. All ISA tested in this experiments showed a fairly good effects to prolong the days to hybrid-pod abscission up to 20 days after hybridization. Cytrabine, procarbazine(N-methylhydrazine) and mitomycine C were approved to maintain the pod-shell development up to 60 days after the cross. But the ovule development was seemed to be continued only up to 40 days after cross even with the ISA treatment. No significant dosage effects on the hybrid pod abscission was observed but the optimum dosage measured at 65 days after the cross was 4,000 ppm, 20 ppm and 100 ppm for cytarabine, mitomycine C, and procarbazine, respectively.

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키워드

Soybeans Interspecific hybridization Immunosuppressants Immunosuppressive agents