Polyploidy induction in the crandon clone of Populus alba × P. grandidentata by colchicine treatment

• 발행기관: 한국육종학회
• 저자명: 전영우(Young Woo Chun) Richard B. Hall
• 간행물 정보: 『한국육종학회지』Vol.24 No.2, 141~150쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1992.06.30

영문 초록

Crandon clone of Populus alba×P. grandidentata was treated with various colchicine concentrations and treatment durations to determine the best method for inducing polyploidy through in vitro treatment of axillary buds. Colchiploid shoots were only produced from in vivo, but not from in vitro colchicine treatments. Most colchiploid shoots originated from buds that were treated 24 h. duration with 1% colchicine solution and 48 h. duration with 2% colchicine solution. Twelve colchiploid shoots produced from in vivo colchicine treatment continued to exhibit the chimera leaf shape after cuttings and have mixed chromosome numbers, 2n (38) and 4n (76). The adventitious shoots produced from chimeric leaf discs through in vitro culture appeared either normal or colchiploid leaf shape with thicker stem diameter. The colchiploid adventitious shoots had the mixed 2n-4n chromosome numbers.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

적요

REFERENCES

키워드

Populus Colchiploid Tetraploid Colchicine Chimera