油菜葯培養의 胚狀體誘起方法 改善

• 영문명: Improvement of embryoid inducing method in anther culture of brassica napus
• 발행기관: 한국육종학회
• 저자명: Jung Il Lee(李正日) Yong Hwan Park(朴用煥) Chung Heon Park(朴忠獻) Jin Ki Bang(方鎭淇)
• 간행물 정보: 『한국육종학회지』Vol.19 No.4, 10~15쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1987.12.30

영문 초록

Rape(Brassica napus) anthers were cultured for the improvement of embryoid induction. Modified B₅ medium was more effecient than modified MS medium for embryoid induction. In case of temperature treatment of anther prior to culture, high temperature at 35℃ for 2days was better than low temperature to improve embryoid induction. Media containing brassinolide or charcocal were not effective for embryoid induction of rape but medium supplemented with proline Ig/ℓ was favourite. Centrifuge prior to anther inoculation was improved embryoid induction at 1,000 G for 30 minutes and the frequency of embryoids obtained was about 1.5%. It was very low in order to apply breeding program. Thus, it was considered that microspore culture was more suitable than anther culture.

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材料 및 方法

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