rs2 유전자 마커를 이용한 Stachyose 저함량 콩 계통 선발

• 영문명: Selection of Low Stachyose Content of Soybean Line Using rs2 Gene Marker
• 발행기관: 한국육종학회
• 저자명: 최상우(Sang Woo Choi) 한성진(Sung Jin Han) 강동휘(Dong Hui Kang) 김진아(Jin A Kim) 이수진(Su Jin Lee) 정종일(Jong Il Chung)
• 간행물 정보: 『한국육종학회지』Vol.49 No.4, 318~323쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.12.30

영문 초록

Soybean cultivars with genetically low levels of stachyose enhance the utilization of soybean in food as well as feed uses. The objective of this research is to obtain the information on indirection selection of soybean lines with low stachyose content using DNA marker based on RS2 (rs2) gene. Two genetic populations were developed from the crosses of three parents (116-13 parent : low stachyose content, PI417227 and PI506903 parents: normal stachyose content). Twenty F 2 plants of RS2_ genotype and twenty F 2 plants of rs2rs2 genotype from each populations were harvested. Content of stachyose was detected by HPLC. Stachyose contents (g/kg) of 116-13, PI417227, PI506903 parents were 3.7, 23.7, and 17.8, respectively. In population 1, stachyose content 20 F 2 plants with RS2_ genotype was 14.8 – 24.1 and stachyose content 20 F 2 plants with rs2rs2 genotype was 2.1 – 4.7. In population 2, stachyose content 20 F 2 plants with RS2_ genotype was 12.4 – 19.7 and stachyose content 20 F 2 plants with rs2rs2 genotype was 2.1 – 5.0. Mean difference between RS2_ genotype and rs2rs2 genotype in population 1 and 2 was highly significant. From this results, selection of genetic lines with low stachyose content by DNA marker based on RS2 (rs2) gene will be possible.

목차

서 언

재료 및 방법

결과 및 고찰

키워드

Stachyose rs2 gene Marker Selection Soybean