SSR마커를 이용한 국내육성 포도 품종의 다양성과 품종 판별

• 영문명: Genetic Diversity and Identification of Korean Grapevine Cultivars using SSR Markers
• 발행기관: 한국육종학회
• 저자명: 조강희(Kang-Hee Cho) 배경미(Kyung-Mi Bae) 노정호(Jung Ho Noh) 신일섭(Il Sheob Shin) 김세희(Se Hee Kim) 김정희(Jeong-Hee Kim) 김대현(Dae-Hyun Kim) 황해성(Hae-Sung Hwang)
• 간행물 정보: 『한국육종학회지』Vol.43 No.5, 422~429쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.10.30

영문 초록

This study was conducted to investigate the genetic diversity and to develop a technique for cultivar identification using SSR markers in grapevine. Thirty Korean bred and introduced grapevine cultivars were evaluated by 28 SSR markers. A total of 143 alleles were produced ranging from 2 to 8 alleles with an average of 5.1 alleles per locus. Polymorphic information contents (PIC) were ranged from 0.666 (VVIp02) to 0.975 (VVIn33 and VVIn62) with an average of 0.882. UPGMA (unweighted pair-group method arithmetic average) clustering analysis based on genetic distances using 143 alleles classified 30 grapevine cultivars into 7 clusters by similarity index of 0.685. Similarity values among the tested grapevine cultivars ranged from 0.575 to 1.00, and the average similarity value was 0.661. The similarity index was the highest (1.00) between Jinok and Campbell Early , and the lowest (0.575) between Alden and Narsha . The genetic relationships among the 30 studied grapevine cultivars were basically consistent with the known pedigree. The three SSR markers sets (VVIn61, VVIt60, and VVIu20) selected from 28 primers were differentiated all grapevine cultivars except for Jinok and Campbell Early . Five cultivars ( Narsha, Alden , Dutchess , Pione , and Muscat Hamburg ) were identified by VVIn61 at the first step. Then 21 cultivars including Hongsodam by VVIt60 at the second step and 2 cultivars ( Heukbosuck and Suok ) by VVIu20 at the third step were identified. These markers could be used as a reliable tool for the identification of Korean grapevine cultivars.

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키워드

Grapevine Cultivar Identification SSR