기체크로마토그래피법에 의한 티아민 분석

• 영문명: Determination of Thiamin by Gas-chromatography
• 발행기관: 대한약학회
• 저자명: 유지상(Ji Sang Yoo) 문동철(Dong Cheul Moon) 홍성화(Sung Hwa Hong) 한건(Kun Han) 김박광(Bak Kwang Kim)
• 간행물 정보: 『약학회지』제33권 제3호 (1989년), 156~160쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1989.06.25

영문 초록

A gas-chromatographic determination method of thiamin which use a quantitative cleavage of thiamin to 4-methyl-5-(2-hydroxyethyl)thiazol[I] and solvent extraction of the analyte prior to GC injection was modified. A column chromatographic procedure using a reversed phase, high capacity solid phase cartridge was applied to the clean-up of the analyte. Thiazol derivative[I] was quantitatively recovered upon the column method. Acetanilide, an internal standard, has a good recovery through the analytical procedure. The method has analytical precision of 2% or less in the coefficient of variation.

키워드

Thiamin gas-chromatographic determination column clean-up acetanilide