전기자극이 체중 부하를 제거한 신생 흰쥐 척수조직의 NT-3 및 MAP2 발현에 미치는 영향

• 영문명: Influence of Electrical Stimulation on NT-3 and MAP2 Expression of Neonatal Rat Spinal Cord During Suspension Unloading
• 발행기관: 한국전문물리치료학회
• 저자명: 구현모(Hyun-mo Koo) 이선민(Sun-min Lee)
• 간행물 정보: 『한국전문물리치료학회지』한국전문물리치료학회지 제14권 제2호, 11~20쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2007.06.30

영문 초록

The development of neonatal neuromuscular system is accomplished by the functional interaction be- tween the spinal neurons and its target cells, skeletal muscle cells, and the intrinsic and extrinsic factors affecting this process. The aim of this study was to identify the effect of suspension unloading (SU) and neuromuscular electrical stimulation (NMES) upon the development of the neonatal spinal cord. For this study, the neonatal rats were randomly divided into three groups: a control group, an experimental group Ⅰ, and an experimental group II. The SU for experimental group Ⅰ and II was applied from postnatal day (PD) 5 to PD 30, and the NMES for experimental group II was applied from PD 16 to PD 30 using NMES that gave isometric contraction with 10 Hz for 30 minutes twice a day. In order to observe the ef-fect of SU and NMES, this study observed neutrophin-3 (NT-3) and microtubule associated protein 2 (MAP2) immunoreactivity in the lumbar spinal cord (LA-5) at the PD 15 and PD 30. The results are as follows. At PD 15, lumbar spinal cord of experimental group Ⅰ and II had significantly lower NT -3 and MAP2 immunoreactivity than control group. It proved that a microgravity condition restricted the spinal development. At PD 30, lumbar spinal cord of control group and experimental group II had significantly higher NT-3 and MAP2 immunoreactivity than experimental group Ⅰ. It proved that the NMES facile-tated the spinal development by spinal cord-skeletal muscle interaction. These results suggest that weight bearing during the neonatal developmental period is essential for the development of neuromuscular development. Also, the NMES on its target skeletal muscle can encourage the development of the spinal cord system with a full supplementation of the effect of weight beating, which is an essential factor in neonatal developmental process.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 결과

Ⅳ. 고찰

Ⅴ. 결론

인용문헌

키워드

Microtubule associated protein 2 Neuromuscular electrical stimulation Neutrophin-3 Spinal cord Suspension unloading