Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis in Human Osteosarcoma Cells

• 발행기관: 대한해부학회
• 저자명: Ji-Hak Min Do-Kyun Kim Hyun-Ho Kwak Gyoo_Cheon Kim Seung-Eun Lee In-Ryoung Kim Chul-Hoon Kim Bong-Soo Park
• 간행물 정보: 『대한해부학회지』제42권 제4호, 245~256쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2009.12.31

영문 초록

Chios gum mastic(CGM) is a resin produced from the stem and leaves of Pistiacia lentiscus L var chia, a plant which grows only on chios Island in Greece. CGM has been used for manu centuries as a dietary supplement and folk medicine for stomach and duodenal ulcers in many Mediterranean countries and is also known to induce cell cycle arrest and apoptosis in some cancer cells. This study was undertaken to investigate the alteration of the cell cycle and induction of apoptosis by CGM treatment on human osteosarcoma(HOS) cells. The viability and the growth inhibition of HOS cells were assessed by the MTT assay and clonogenic assay respectively. The hoechst staining, TUNEL assay and DNA electrophoresis were conducted to observe the HOS cells undergoing apoptosis. HOS cells were treated with CGM, and Western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, mitochondrial membrane potential change and proteasome activity were conducted. CGM treatment of HOS cells was found to result in a dose-and time-dependent decrease in cell viability, a dose-dependent inhibition of cell growth, and apoptotic cell death. Tested HOS cells also showed several lines of apoptotic manifestation and G1 arrest in cell cycle progression. In summary, this study clearly demonstrated that CGM induces G1 cell cycle arrest via the modulation of cell cycle-related proteins, and apoptosis via proteasome, mitochondrial and caspase cascades in HOS cells. Therefore, our data provide the possibility that a natural product, CGM could be considered as a novel therapeutic strategy for human osteosarcoma.

키워드

Chois gum mastic(CGM) Apoptosis Cell cycle arrest Human osteosacoma cells(HOS cells)