Preconditioning enhances neurotrophic factor expression in rat bone marrow mesenchymal stem cells for neuroregenerative applications

• 발행기관: 대한해부학회
• 저자명: Sareh Pandamooz Mohammad Ghasemian Zahra Jamali Maryam Hassanpour Mohammad Javad Mokhtari Shahrokh Zare Mehdi Dianatpour Mohammad Reza Jafarzadeh Shirazi Mohammad Saied Salehi
• 간행물 정보: 『Anatomy and Cell Biology』Vol.58(2), 284~293쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

Stroke is a leading cause of mortality and disability in adults worldwide. Among the various treatment strategies,cell-based therapies have gained considerable attention due to their regenerative potential. Enhancing the efficacy of stemcells is critical to improve therapeutic outcomes. Dimethyl fumarate (DMF) is one of the drugs that has been recognized forits ability to modulate the paracrine effects of stem cells. This study aimed to investigate the effect of different concentrationsof DMF on rat bone marrow mesenchymal stem cells (BM-MSCs). The BM-MSCs viability following treatment with variousdoses of DMF was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and fluoresceindiacetate staining at 24 and 72 hours. After identifying the optimal DMF concentration, BM-MSCs were cultured withselected DMF concentration for 72 hours, and their gene expression profiles of key neurotrophic factors were analyzed usingquantitative real-time polymerase chain reaction. Our findings revealed that 1 μM DMF was the optimal concentrationfor enhancing BM-MSC viability. Treatment with this dose significantly upregulated the expression of brain-derivedneurotrophic factor, nerve growth factor, and neurotrophin-3, highlighting their potential in promoting neuronal supportand regeneration. In contrast, the transcript level of glial-derived neurotrophic factor was significantly reduced, suggesting aselective regulatory effect of DMF on neurotrophic pathways. These findings shed light on the therapeutic promise of DMF inmodulating neurotrophic factor expression in BM-MSCs, offering novel insights into its application in regenerative medicinefor neurodegenerative conditions.

목차

Introduction

Materials and Methods

Results

Discussion

ORCID

Author Contributions

Conflicts of Interest

Funding

References

키워드

Mesenchymal stem cells Dimethyl fumarate Priming Growth factor