Hair follicle stem cell-derived secretome protects astrocytes in an in vitro ischemia/reperfusion model

• 발행기관: 대한해부학회
• 저자명: Fatemeh Jameie Mehdi Dianatpour Mahintaj Dara Zahra Jamali Nasrin Ghorbani Gökhan Ünal Mohammad Saied Salehi Sareh Pandamooz
• 간행물 정보: 『Anatomy and Cell Biology』Vol.58(2), 264~273쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

Ischemic stroke causes significant neuronal and glial cell damage. Recent studies suggest that stem cell-derivedsecretomes may offer therapeutic benefits for neural injuries. This study evaluates the protective effects of hair follicle stemcell (HFSC)-derived secretome on astrocytes subjected to oxygen-glucose deprivation (OGD), an in vitro model of ischemicstroke. In this regard, the primary astrocyte cultures were exposed to OGD conditions for 24 hours, followed by treatmentwith HFSC-derived secretome for 48 hours to create an environment rich in paracrine factors. The neuroprotective effect ofHFSC-derived secretome on injured astrocytes was assessed using MTT assay, apoptosis flow cytometry, and qRT-PCR. TheHFSC secretome mitigated cell death and apoptosis in OGD-induced astrocytes. Additionally, the secretome reduced themRNA expression levels of pro-inflammatory cytokines IL-6, IL-1β, and TNF-α compared to the injured cells. Furthermore,it upregulated the mRNA levels of neurotrophic factors BDNF, and VEGF after OGD in astrocytes. These findings suggestthat the reparative effects of the secretome are associated with astrocyte neuroprotection, anti-inflammatory properties, andanti-apoptotic effects. The neuroprotective effect of HFSC secretome may be associated with the upregulation of neurotrophicand angiogenic factors. Restored astrocytes create a conducive environment for repair, thereby expediting the recovery ofimpaired brain function. This study provides preclinical evidence supporting the potential of HFSC secretome in stroketherapy to improve treatment outcomes of patients who suffered from ischemic stroke.

목차

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키워드

Neural crest Culture media Conditioned Ischemic stroke Hair follicle-associated pluripotent