25-OH-vitamin D3 RIA 시약별 결과값 비교분석 및 유효성 평가

• 영문명: Comparative Analysis and Validation of Results by 25-OH-vitamin D3 RIA Reagents
• 발행기관: 대한핵의학기술학회
• 저자명: 임슬기(Seul Gi Lim) 이영지(Young Ji Lee) 이선호(Sun Ho Lee)
• 간행물 정보: 『핵의학기술』Vol.29 No.1, 3~8쪽, 전체 6쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2025.05.31

국문 초록

Purpose: When vitamin D3 is deficient in the body, it leads to osteoporosis, fractures at high risk. In addition, vitamin D3 deficiency is also associated with the pathogenesis of certain cancers and other diseases including multiple sclerosis, type 1 diabetes. In fact, the number of prescriptions for vitamin D3 requested to the Department of Nuclear Medicine at Asan Medical Center was increased. As such, the examination of vitamin D3 in the Department of Nuclear Medicine is a test that provides clinically meaningful indicators. However, due to the difficulty of stopping the production of the previously used vitamin D3 RIA reagent (DIA source ImmunoAsays®, Belgium) to measure vitamin D3, a validity assessment experiment was conducted on vitamin D3 RIA reagent (Shinjin, Korea). Materials and Methods: For about two months from February 1 to March 31, 2023, the result values of the two reagents were analyzed for various concentrations of vitamin D3 samples (N=45) requested to the our clinic. The effectiveness of the alternative reagent was evaluated using the correlation coefficient between the existing and alternative reagents. We also measured the correlation coefficient between the 25-OH-vitamin D (Total) and the vitamin D3 reagents and the correlation with high performance liquid chromatography/mass spectrometry (HPLC) was analyzed. In addition, we caculated the difference(%) of the target value of the DEQAS(vitamin D External Quality Assessment Scheme, UK). The inter assay, intra assay, analytical sensitivity and recovery were evaluated for alternative reagents. Results: The correlation coefficient between the existing and alternative vitamin D3 kits was close to 1 with R2=0.7556(p<0.001) and there was good correlation between the 25-OH-vitamin D(Total) kit and alternative vitamin D3 kit with R2=0.9409(p<0.001). The correlation coefficient between the alternative vitamin D3 kit and HPLC measurement was close to 1 with R2=0.8384(p<0.001). In case of difference(%) assessment with DECAS reagents, the alternative vitamin D3 kits showed 7.17, 10.90, 15.10, 20.63, 20.52 in January 2022 and 1.02, -0.42, -0.04, -13.62, -12.50 in July2022 respectively. As a result of measuring the intra assay, the Coefficient of Variation (CV) value of the alternative vitamin D3 kit were 13.35, 7.73, 9.28 in low, medium, high concentration respectively. In case of the intra assay, CV value were 14.65, 6.10, 7.25 in low, medium, high concentration respectively. Except the CV value of low concentration, they were acceptable. The analytical senstivity was 0 ng/mL. But the recovery rate(%) performed not good enough at 30~162. Conclusion: Alternative reagents showed high correlation with existing reagents (p<0.001), 25-OH-vitamin D (Total), and high correlation with HPLC (p<0.001) and due to its high consistency with DEQAS materials, it is thought that reagents can be replaced and provide quantitatively reliable results. Considering that the normal level of the reference value of the alternative reagent is 30 to 150 ng/mL, it does not seem to be a major clinical problem, but further experiments need to be checked. Based on this, it is thought that even if the reagent of vitamin D3 is changed, accurate test data can be continuously provided.

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키워드

25-OH-vitamin D3 RIA reagents Correlation coefficient