신속 요소 분해 효소 검사의 시험액 내 미네랄 이온 농도 변화가 발색반응 속도에 미치는 영향: Helicobacter pylori 감염의 연관성

• 영문명: Sodium, Potassium, Calcium and Magnesium Affect Colorimetric Changes of Rapid Urease Test: Implication on Helicobacter pylori Infection
• 발행기관: 대한약학회
• 저자명: 윤소미(Somy Yoon) 이미진(Mijin Lee) 조영창(Young-Chang Cho) 김항건(Hangun Kim)
• 간행물 정보: 『약학회지』제69권 제2호(2025년), 174~179쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2025.04.30

국문 초록

Helicobacter pylori is a Gram-negative bacterium that is found in the stomach, infection of which is strongly correlated with the occurrence of chronic gastritis, gastric ulcers, and gastric cancer. To endure in acidic environment of mucus layer of stomach, H. pylori uses several ways and secretion of urease to neutralize gastric acid by producing ammonia from urea is among the key features. With this, rapid urease test was widely used to detect H. pylori infection using biopsy specimens. In this study, we explored several supplements that can facilitate a colorimetric change of rapid urease test to shorten the necessary time to confirm a test result. As a result, passive lysis of bacterial barrier using lysozyme, NP-40, or SDS did not facilitate the reaction and even showed inhibitory effect probably due to the inactivation of urease. Addition of low concentration of adenosine tri-phosphate slightly enhances the reaction. Surprisingly, supplementation of sodium and potassium ions prompts the completion of the reaction. In contrast, supplementation of calcium and magnesium ions suppresses the reaction. These results suggest that above ions affect necessary diagnosis time for rapid urease test in detection of H. pylori and implicate the mechanistic insight how dietary supplementation of inorganic ions such as high-salt diet promotes the infection of H. pylori.

목차

서 론(Introduction)

방 법(Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

감사의말씀(Acknowledgment)

Conflict of Interest

References

키워드

Helicobacter pylori Mineral Rapid urease test Urease activity