GWAS Analysis to Identify Candidate Genes Related to Phosphorus Deficiency Tolerance by GWAS in Rice

• 영문명: GWAS Analysis to Identify Candidate Genes Related to Phosphorus Deficiency Tolerance by GWAS in Rice
• 발행기관: 한국육종학회
• 저자명: Chuluuntsetseg Jadamba Jeong Man Kim Hye-Jee Lee Eun Gyul Kim Soo-Cheul Yoo
• 간행물 정보: 『Plant breeding and biotechnology』Vol.12, 82~97쪽, 전체 16쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2024.03.30

국문 초록

Rice yield is severely affected by phosphorus (P) deficiency, and plants have evolved various strategies to cope with this limitation. While some rice genotypes are adapted to low phosphate (Pi) availability, others remain sensitive to Pi deficiency. In this study, we conducted a genome-wide association study (GWAS) using a hydroponically cultivated population of 190 North Korean (NK) rice plants to identify genes associated with phosphorus use efficiency (PUE) and Pi deficiency tolerance. The rice plants were grown in Yoshida nutrient media with either full (10 mg/L) or low-P (1 mg/L) concentrations for 40 days. The phenotypic response to Pi deficiency was assessed at the seedling stage, followed by an evaluation of eight agricultural traits: chlorophyll content (SPAD), shoot length (SL), shoot fresh weight (SFW), shoot dry weight (SDW), root fresh weight (RFW), root dry weight (RDW), and tiller number (TN). The GWAS analysis revealed a total of 166 significant lead SNPs, with six located near known genes for Pi deficiency tolerance: OsTre6P and OsPT3 for RL, OsGH3.12 for SPAD, OsCPK30 for SL, OsWRKY74 for RSL, and OsPT10 for RSL and RRFW. An additional six lead SNPs were identified as novel genes. The haplotypes of 12 candidate genes showed significant differences in the phenotypic values of the corresponding traits. In conclusion, both known and novel genes identified in this GWAS have significant impacts on Pi deficiency tolerance in the NK rice population.

키워드

Rice Phosphorus Pi deficiency Genome-wide association study

참고문헌

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• Scientific Reports
• Plant Physiol
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• Annu Rev Plant Biol
• Science
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• Plant Physiol
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• Rice
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• PloS One
• Front Plant Sci
• Plant Cell Environ