Estradiol 검사 시 방사성 추적자의 희석시간에 대한 고찰

• 영문명: The Study on the Dilution Time of Radioactive Tracer in Estradiol Measurement
• 발행기관: 대한핵의학기술학회
• 저자명: 이혜연(Hae Yeon Lee) 서한경(Han Kyung Seo) 장이선(Yi Sun Jang) 김희정(Hee Jeoung Kim)
• 간행물 정보: 『핵의학기술』Vol.21 No.2, 44~48쪽, 전체 5쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2017.11.30

영문 초록

Purpose Estradiol (E2) is a steroid hormone mainly produced in women and is a useful indicator for diagnosis of gynecological diseases, menstrual cycle, menopause, and precocious puberty. E2 measurement is performed by diluting the ¹²⁵I radioactive tracer and tracer buffer in the kit. However, It was not precisely specified when the period of tracer is available after activating. The purpose of this study was to determine the appropriate dilution time based on the measurement value with dilution time. Materials and Methods From December 2016 to February 2017, 60 E2 samples with concentrations ranging from 8 to 4577 pg/mL were divided into low, medium, and high concentrations. Dilution of the ¹²⁵I tracer was performed on a 230 RPM agitator for 30 minutes, 1 hour 30 minutes, and 2 hours 30 minutes, respectively. 24 hour dilution was gently shaken and refrigerated. To verify the difference and significance of the results according to the dilution time, a test of normality was performed using SPSS 18.0 and analyzed by Kruskal-Wallis test. The measured value according to the dilution time was compared with the interquartile range of the absolute error. Results The results of Kruskal-Wallis test were not significant (P>0.05). Measurement results are showed as interquartile range of absolute error. At low concentration, it is 0.052 between 1 hour 30 minutes and 2 hours 30 minutes, and 0.105 between 30 minutes and 1 hour 30 minutes. At medium concentration, 0.062 between 30 minutes and 1 hour 30 minutes, and 0.038 between 1 hour 30 minutes and 2 hours 30 minutes. At high concentration, it is 0.029 between 1 hour 30 minutes and 2 hours 30 minutes, and 0.06 between 2 hours 30 minutes and 24 hours. Conclusion There were no statistically significant differences. However, the change in the measured value is the smallest between 1 hour and 30 minutes to 2 hours and 30 minutes. Therefore, we recommend diluting time between 1 hour 30 minutes and 2 hours 30 minutes.

목차

서론

대상 및 방법

결과

결론 및 고찰

요약

참고문헌

키워드

Estradiol Radioactive tracer Dilution time