Insulin 측정용 방사면역측정법 시약의 평가

• 영문명: The Evaluation of Radioimmunoassay kits for Insulin
• 발행기관: 대한핵의학기술학회
• 저자명: 신용환(Yong Hwan Shin) 김윤현(Yun Hyun Kim) 이일규(Il Kyu Lee) 김지영(Ji Young Kim) 석재동(Jae Dong Seok) 신숙희(Suk Hee Shin)
• 간행물 정보: 『핵의학기술』Vol.16 No.2, 149~155쪽, 전체 7쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2012.10.30

영문 초록

Purpose : Serum insulin levels are useful indicator which of reflecting the function of insulin secretion in pancreatic β cell and diagnosis of diabetes, differentiating the cause of impaired glucose tolerance. Insulin measurement kits have shown some differences in many ways such as test methods as well as quality control. The purpose of this study was to evaluate the diagnostic performance of seven manufacturing companies commercial kits. Materials and Methods : The values of insulin measured by three manufacturing companies (Biosource, Siemens, TFB) with 59 samples in August 2009 were compared with those measured by four manufacturing companies (Immunotech, Izotope, BNIBT, Cisbio) with 68 samples in December 2011. We evaluated precision, recovery rate, dilution test and correlation of serum insulin measurement using seven manufacturing company kits. Statistical program SPSS 12.0 was used for the verification of results. Results : The coefficients variation of the precision on all seven different kits were showed within 5.0%. Recovery rate of Biosource, Siemens, TFB kits on three different levels showed 94.2~103.7%, 99.0~104.6%, 99.7~ 107.6% respectively. Immunotech, Izotope, BNIBT, Cisbio were 93.5~99.1%, 91.4~99.1%, 99.2~131.0%, 84.8~102.3% respectively. There was strong correlation between the measurement of insulin by Biosource kit and that by two commercial kits, Siemens (R2=0.96), TFB (R2=0.99). There was good correlation between the measurement of insulin by TFB kit and that by three commercial kits, Immunotech (R2=0.97), Izotope (R2=0.96), Cisbio (R2=0.97). In the dilution test performed with more than 200 μIU/ml high concentration samples, samples with diabetes correctly was measured in all seven manufacturing kits. However, as measured with insulinoma samples TFB, Siemens, Izotope, Cisbio kits were correctly measured , but Biosource and Immunotech kits were measured 47.4 μIU/ml, 72.3 μIU/ml, respectively. Conclusion : Serum Insulin radioimmunoassay kits were showed excellent precision, correlation and good recovery rate. However, some kits were not measured correctly in the high concentration insulin values. when selecting a kit should be considered many factors that cost effectiveness, compatible for automation equipment, high performance kit, the environment for each laboratory such as reaction time and reporting time.

목차

서론

대상 및 방법

결과

연구의 제한

고찰 및 결론

요약

REFERENCES

키워드

Radioimmunoassay Precision Recovery Correlation Dilution test Insulin