고정용 팁을 사용하는 Automated Liquid-Handling System의 RIA적용의 평가

• 영문명: Evaluation of an Automated Liquid-Handling System with Fixed Pipetting Tips in Radioimmunoassay
• 발행기관: 대한핵의학기술학회
• 저자명: 신선영(Sun Young Shin) 김희선(Hee Sun Kim) 이현주(Hyun Ju Lee) 장현영(Hyun Young Jang) 우재룡(Jae Ryong Woo) 이호영(Ho Young Lee)
• 간행물 정보: 『핵의학기술』Vol.14 No.2, 190~196쪽, 전체 7쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2010.10.30

영문 초록

Purpose: This study was undertaken to investigate the feasibility of using automated liquid-handling systems equipped with reusable fixed tips in Radioimmunoassays and to demonstrate that the use of an automated pipetting instrument can decrease in the typical daily workload. Materials and Methods: The precisions of the automated pipettor and the manual pipettor were determined gravimetrically (n=30). A total of 30 specimens for HBs Ag were repeatedly pipetted (three replicates) with the automated pipettor and then retested. PSA samples were simultaneously pipetted with either the automated pipettor or the manual pipettor and then analyzed (n=40). Sample carryover test assessed for CA19-9, AFP and HCG when the automated pipettor was used. Pipetting speed of the automated pipettor and the manual pipettor were compared by evaluation of each workload. Results: The precisions coefficients of variation (CV) were 2.1% for the automated pipettor and 1.6% for manual pipettor. The mean cpm and CV for each group of replicates were 41,203 cpm and 3.7% for HBs Ag positive specimens, and 99 cpm and 7.9% for HBs Ag negative specimens, respectively. PSA results showed no significant differences between automated pipettor and manual pipettor (p=0.15, r=0.999). Carryover for CA19-9, AFP and HCG analytes was <0.1 ppm or below the assay limit of detection. Pipetting speed was significantly improved by using the automated instrument. Conclusion: There was no evidence that the use of an automated pipettor adversely affected any of the performance characteristics of the assay. Indeed, routine use of the Tecan automated pipettor has resulted in a decrease in the typical daily workload.

목차

서론

실험재료 및 방법

결과

결론

요약

REFERENCES

키워드

Radioimmunoassay Automated liquid-handling system Precision Carryover Workload