서울아산병원의 적혈구 표지 방법에서 교반 시간 차이에 따른 표지 효율의 분석

• 영문명: Analysis of Labelling Efficiency According to Differences of Rotating Time in a Asan Medical Center (AMC) RBC Labelling Method
• 발행기관: 대한핵의학기술학회
• 저자명: 정은미(Eun Mi Chung) 정우영(Woo Young Jung) 류재광(Jae Kwang Ryu) 심동오(Dong Oh Shim) 이영희(Yeong Hee Lee)
• 간행물 정보: 『핵의학기술』Vol.14 No.1, 90~93쪽, 전체 4쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

Purpose: In our nuclear medicine department, we suggested AMC RBC labeling method improved by modifying a part of existing modified in-vitro method to raise the efficiency of ⁹⁹ᵐTc-RBC labeling. However, it needs to be more additional time and efforts than existing modified in-vitro method because the AMC RBC labeling method has to carry out the centrifugal separation process for 3~5 minutes. Therefore, in this study, we conducted researches to aim to maintain stable labeling effects and supplement a problem about additional time by reducing rotating time when labeling ⁹⁹ᵐTc-RBC. Materials and Methods: This research has been conducted the object of 30 patients who examined study using ⁹⁹ᵐTc-RBC and agreed to this research at our hospital from May 2009 to September 2009. We made 4 blood samples which consisted of ACD 1 cc along with 5 cc blood from each patient and used the AMC RBC labeling method. At this moment, each labeling efficiency was calculated by different rotating time 5 min, 10 min, 15 min, and 20 min and then we compared differences. Results: As a result, When comparing the ⁹⁹ᵐTc-RBC labeling method efficiency by using the AMC RBC labeling method which differents from rotating time, each labeling efficiency were 92.3±5.0 % in 5 min, 95.9±5.0 % in 10 min, 97.4±4.9 % in 15 min and 97.7±4.8 % in 20 min. We analyzed differences of the labeling efficiency from change of rotating time by using an one-way ANOVA and verified that in Duncan method. There was relatively efficiency low in 5min rotating time and no statistically significant change in over. Conclusions: When comparing a existing method, the AMC RBC labeling method which goes through the centrifugal separation process again offers more favorable condition to combine RBC with ⁹⁹ᵐTc04 - by eliminating an plasma ingredient. When using the modified in-vitro method, we have almost 20 min to rotate to acquire stable labeling efficiency. But, when using the AMC RBC labeling method, we acquire labeling efficiency well what we want within only 10 min to rotate. Decrease of rotating time can complement the AMC RBC labeling method which goes through the centrifugal separation process again and also provide more rapid study such as G-I bleeding study due to fast labeling.

목차

서론

실험재료 및 방법

결과

고찰 및 결론

요약

REFERENCES

키워드

Rotating Time ⁹⁹ᵐTc-RBC AMC RBC Labelling method Modified in-vitro method