Phytate를 이용한 감시림프절 검사 시 Filter의 유용성 평가

• 영문명: Evaluation on the Usefulness of Filter in Sentinel Lymphoscintigraphy Using ⁹⁹ᵐTc-Phytate
• 발행기관: 대한핵의학기술학회
• 저자명: 정영환(Yeong Hwan Jeong) 서한경(Han Kyung Seo) 심철민(Cheol Min Shim) 임성동(Seong Dong Lim) 한동현(Dong Hyeon Han) 박영순(Yung Sun Park) 김동윤(Dong Yun Kim)
• 간행물 정보: 『핵의학기술』Vol.14 No.1, 35~39쪽, 전체 5쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

Purpose: The aim of this study was to investigate distribution of particle size in phytate kit and compare filtered method with non-filtered method using 200 nm filter for sentinel lymphoscintigraphy (SLS). Materials and Methods: Five phytate kit of having the same available period was measured by particle size analyzer. For in-vivo experiment, ⁹⁹ᵐTc-phytate was injected intradermally at both foot to perform lymphoscintigraphy. Imaging was acquired at 1hour after injection. Region of interest (ROI) was drawn in inguinal and background area for analysis. RAW 264.7 cells (Murine macrophage cell) were prepared for measurement of celluar uptake as a representative of macrophages. Paired t-test was performed using SPSS (SPSS Inc, USA) for statistical analysis. Results: The size of most particle in Techne phytate kit was distributed in 130~650 nm(90.5 %). In-vivo study, the ROI analysis showed similar result between filtered and non-filtered sample, and the numerical value of count/pixel were 58.3±5.97 and 60.2±4.88. In-vitro study, cellular uptake study also showed no difference between filtered and non-filtered sample by gamma counting. Conclusion: The present study demonstrates that there was no meaning of 200 nm filtered method for SLS using ⁹⁹ᵐTc-phytate.

목차

서론

실험재료 및 방법

결과

고찰

요약

REFERENCES

키워드

Phytate Sentinel Lymphoscintigraphy 200 nm Filter