Chlorogenic acid alleviates the reduction of 14-3-3 protein in the cerebral cortex of stroke animal model

• 영문명: Chlorogenic acid alleviates the reduction of 14-3-3 protein in the cerebral cortex of stroke animal model
• 발행기관: 한국예방수의학회
• 저자명: Murad-Ali Shah Yeung Bae Jin Myeong-Ok Kim Phil-Ok Koh
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.47, No.3, 137~145쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2023.09.30

영문 초록

Ischemic stroke causes severe neuronal damage. Chlorogenic acid is a phenolic substance present in fruits and coffee. It also exerts neuroprotective effects against various brain injuries. The 14-3-3 family protein perform a variety of functions including metabolism, signal transduction, cell differentiation, and apoptosis. The purpose of this study is to investigate whether chlorogenic acid regulates the expression of 14-3-3 protein in stroke animal models. Ischemic stroke was induced by middle cerebral artery occlusion (MCAO) surgery. Phosphate buffered saline (PBS) or chlorogenic acid (30 mg/kg) were intraperitoneally injected to adult male rats 2 h before MCAO surgery. Adhesive-removal test was performed 24 h after MCAO surgery and cerebral cortical tissues were collected for further study. MCAO damage caused severe neurological impairment and chlorogenic acid treatment ameliorated this disorder. Our proteomic approach showed a decrease in 14-3-3 expression in MCAO animals with PBS. The decrease in 14-3-3 expression alleviated in MCAO animal with chlorogenic acid. We confirmed changes in various 14-3-3 protein isoforms, including beta/alpha, zeta/delta, gamma, epsilon, eta, and tau through reverse transcription-PCR. These results explained that chlorogenic acid regulates the expression of 14-3-3 protein in MCAO-induced cerebral ischemia. 14-3-3 is considered to be an important protein for cell survival through binding to pro-apoptotic proteins. The maintenance of 14-3-3 levels is an important event in neuroprotection against ischemic injury. Therefore, we can demonstrate that the 14-3-3 protein contributes to the neuroprotective effect of chlorogenic acid in stroke animal models.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

Acknowledgments

REFERENCES

키워드

14-3-3 Chlorogenic acid Middle cerebral artery occlusion Stroke