닥나무 속 식물의 엽록체 유전체 기반 InDel 마커의 개발

• 영문명: Development of Chloroplast Genome-based Insertion/Deletion Markers in the Genus Broussonetia
• 발행기관: 한국자원식물학회
• 저자명: 이은지 김윤아 이미선 김주혁 최용규 김정성 신창섭 이이
• 간행물 정보: 『한국자원식물학회지』제36권 제4호, 290~298쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.08.31

영문 초록

Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia.

목차

서언

재료 및 방법

결과

고찰

적요

사사

Conflicts of Interest

References

키워드

Broussonetia kazinoki Broussonetia monoica Broussonetia papyrifera Genotyping Next generation sequencing