Allelic Diversity at Protein Disulfide Isomerase Like 1-1 (PDIL1-1 ) Gene is Associated with Amylose Content in Japonica Rice

• 영문명: Allelic Diversity at Protein Disulfide Isomerase Like 1-1 (PDIL1-1 ) Gene is Associated with Amylose Content in Japonica Rice
• 발행기관: 한국육종학회
• 저자명: Cheryl Adeva 강주원 심규찬 Ngoc Ha Luong 이현숙 이종희 안상낙
• 간행물 정보: 『Plant breeding and biotechnology』Vol.11 No.1, 56~68쪽, 전체 13쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.03.30

영문 초록

Amylose content is a key factor affecting the eating and cooking qualities of rice. In our previous study, protein disulfide isomerase like 1-1 (PDIL1-1) on chromosome 11 was a candidate gene for amylose content (AC) trait. Based on whole genome sequencing, polymorphisms were identified between Dodamssal and Hwayeong on PDIL1-1. In this study, the association of PDIL1-1 on AC was characterized. Haplotype analysis of 79 KNU accessions highlighted the presence of allelic patterns identifiable by the sequence variants between Dodamssal and Hwayeong. Identified SNPs and InDel were used to develop gene-based molecular markers for PDIL1-1. The 29 F2 plants and 160 Korean japonica cultivars were classified into two and three groups, respectively, based on the G/A SNP at position -4973180 and ATTCG/G at position -4974161. Our findings revealed that the G/A SNP at position -4973180 of PDIL1-1 plays a role in regulating the AC in japonica rice. This suggested that PDIL1-1 would be useful for fine-tuning the rice AC. To our knowledge, no studies of the allelic variation of the PDIL1-1 gene regulating AC have been reported worldwide. Furthermore, no research had reported the development of PDIL1-1 gene-based molecular markers.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

CONCLUSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Endosperm storage protein 2 Protein disulfide isomerase like 1-1 Rice Single nucleotide polymorphism