Ascorbate Peroxidase OsAPx1 is Involved in Seed Development in Rice

• 발행기관: 한국육종학회
• 저자명: Yeon Jeong Kim Sung-Il Kim Markkandan Kesavan Jun Soo Kwak Jong Tae Song Hak Soo Seo
• 간행물 정보: 『Plant breeding and biotechnology』Vol.3 No.1, 11~20쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.03.01

영문 초록

Ascorbate peroxidases (APXs) are enzymes that detoxify peroxides such as hydrogen peroxide using ascorbate. They are distributed as isoenzymes in distinct cellular compartments, such as the cytosol, mitochondria and peroxisomes, where they play essential roles in scavenging reactive oxygen species (ROS) and protecting cells against the toxic effects of these species in higher plants, algae, euglena and other organisms. APXs also respond to environmental stresses, such as salinity and drought, and rice ascorbate peroxidase 1 (OsAPx1) participates in salinity tolerance. However, it is still unclear how OsAPx1 is involved in growth and development before and after flowering. Here, we show that OsAPx1 plays an important function in seed development, including fertilization. Proteomic analysis and quantitative RT-PCR showed that protein and mRNA levels of OsAPx1 were much higher in immature seeds than in mature seeds. Plant height and grain size in an Osapx1 mutant were almost the same as those in wild-type plants. However, about 58% of the seeds were aborted in the Osapx1 mutant, although the mutant was capable of normal flowering. Our results suggest that rice ascorbate peroxidase functions both as a regulator of seed development and as a scavenger of ROS.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Ascorbate peroxidase OsAPx1 Seed development Rice