충청남도 해안지역의 비브리오 패혈증균 분포 및 분리주의 특성 분석

• 영문명: Distribution and Characterization of Vibrio vulnificus Isolated in Coastal Areas of Chungcheongnam-do Province
• 발행기관: 한국환경보건학회
• 저자명: 이현아(Hyunah Lee) 고영은(Young-Eun Ko) 최지혜(Jihye Choi) 이다연(Dayeon Lee) 여성순(Seoungsoon Yeo) 박종진(Jongjin Park) 이미영(Miyoung Lee) 최진하(Jinha Choi) 박준혁(Junhyuk Park)
• 간행물 정보: 『한국환경보건학회지』제47권 제5호, 479~485쪽, 전체 7쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2021.10.31

영문 초록

Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.

목차

I. 서 론

II. 재료 및 방법

III. 결과 및 고찰

IV. 결 론

키워드

16s rRNA sequence antibiotic susceptibility Chungcheongnam-do coastal areas Vibrio vulnificus