Enhancing in vitro Growth of Bulbs for Mass Propagation of Lily Germplasm

• 발행기관: 한국자원식물학회
• 저자명: Jae-young Song Young-yi Lee Jung-yoon Yi Jung-ro Lee Mun-sup Yoon
• 간행물 정보: 『한국자원식물학회지』제34권 제1호, 17~22쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2021.02.28

영문 초록

Plants regenerated from in vitro cultures carry chromosomal variations, especially in long-term culture. Reducingthe duration of plant tissue culture is one of the ways to reduce genetic and epigenetic changes. In this study, we reduced theduration of long-term culture and repeat subculture using small bulblets derived from bulb scales in two lily cultivars. Theadventitious bulblets derived from bulb-scale tissue were cultured on three different media containing Murashige and Skoog(MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 ㎎/L IAA and 0.4 ㎎/L BA hormonewith or without Charcoal, respectively. About seven weeks later, the number of newly propagated multiple shoots in the twomedia, A and B media, showed little differentiation. Compared to both media, the number of propagated multiple shootsincreased 5-fold in MS medium containing 0.3 ㎎/L IAA and 0.4 ㎎/L BA hormone without Charcoal (C medium). Thenumber of propagated multiple shoots ranged from 5 to 6 and 4 to 6 with an average of 5 in TropicalPink and GreenStarcultivars, respectively. The flow cytometric measurements indicated no variation in the ploidy level between control and invitro propagated plants.

목차

ntroduction

Materials and Methods

Results and Discussion

키워드

Activated charcoal Bulb Lilium Ploidy Propagation