어성초, 금잔화 추출물의 모유두세포와 지방전구세포에 대한 세포독성 및 발모효과

• 영문명: Cell Viability and Hair Growth Effect of Ethanol Extract from Houttuynia cordata Whole Plant and Calendula arvensis Flower on HDFDPC and 3T3-L1 Cells
• 발행기관: 한국자원식물학회
• 저자명: 진성우(Jin, Seong-Woo) 김경제(Kim, Kyung-Je) 고영우(Koh, Young-Woo) 임승빈(Im, Seung-Bin) 정상욱(Jeong, Sang-Wook) 서경순(Seo, Kyoungsun)
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2018년도 춘계학술발표회, 79~79쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.04.30

영문 초록

Chlorogenic acid is a phenolic compound found in Cudrania tricuspidata fruits. In the present study, the effect of chlorogenic acid on the inhibition of adipogenesis in 3T3-L1 adipocytes was investigated. Cells were stained with Oil red O reagent to detect lipid droplets in adipocytes. The 3T3-L1 cells were lysed and measured for intracellular triglyceride and adipokine by ELISA kit. The protein expression of adipogenesis-related gene was evaluated by Western blot analysis. Chlorogenic suppressed lipid droplet and intracellular triglyceride accumulation in a concentration manner and also decreased secretion of adipokines such as leptin and adiponectin, compared with fully differentiated adipocytes. Treatment of 3T3-L1 cells with chlorogenic acid reduced the protein levels of peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and, CCAAT/enhancer binding proteins alpha ($C/EBP{\alpha}$). This indicates that chlrogenic acid was effective as an anti-obesity agent by repressing the differentiation of 3T3-L1 into adipocytes and inhibiting triglyceridef formation in adipocyte and that it exerts its role mainly through the significant down-regulation of $PPAR{\gamma}$ and $C/EBP{\alpha}$.

키워드

Adipocyte Adipogenesis Adipokine