Anticancer Activity of Sageretia thea Through β-catenin Proteasomal Degradation in Human Colorectal Cancer and Lung Cancer Cells

• 영문명: Anticancer Activity of Sageretia thea Through β-catenin Proteasomal Degradation in Human Colorectal Cancer and Lung Cancer Cells
• 발행기관: 한국자원식물학회
• 저자명: Kim, Ha Na Park, Su Bin Kim, Jeong Dong Jeong, Jin Boo
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2019년도 춘계학술대회, 92~92쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.04.30

영문 초록

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased ${\beta}$-catenin protein level, but not mRNA level in A549 cells. The downregulation of ${\beta}$-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated ${\beta}$-catenin protein, the inhibition of $GSK3{\beta}$ by LiCl did not blocked the reduction of ${\beta}$-catenin by TY-NS-B. In addition, TY-NS-B decreased ${\beta}$-catenin protein in A549 cells transfected with Flag-tagged wild type ${\beta}$-catenin or Flag-tagged S33/S37/T41 mutant ${\beta}$-catenin construct. Our results suggested that TN-NS-B may downregulate ${\beta}$-catenin protein level independent on GSK3${\beta}$-induced ${\beta}$-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer.

키워드

Anticancer activity ${\beta}$-catenin Cancer chemoprevention Cell growth Taxillus yadoriki