Isolation and Characterization of a cDNA(Fp1) Encoding the Iron Storage Protein in Red Pepper(Capsicum annuum L.)

• 발행기관: 한국자원식물학회
• 저자명: Young Ho Kim Young Ok Lee Ill Sup Nou Hee Wan Kang Toshiaki Kameya Takashi Saito Kwon Kyoo Kang
• 간행물 정보: 『Plant Resources』Vol.1 No.1, 13~21쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1998.04.30

영문 초록

A cDNA fragment encoding iron storage protein generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to screen a red pepper cDNA library, cDNA clone was designated as Fpl. Fpl clone contained a 5 nontranslated region of 51bp containing stop codons. Down stream from 5 UTR, an open reading frame of 750bp was observed, followed by a 3 UTR of 272bp. The deduced amino acid sequence of red pepper protein(Fpl) showed 84%, 48% and 36% identity with soybean(SolC), human(HuL-H) and horse spleen (HOS-L) ferritin respectively. Northern blot analysis of root and leaf RNAs, at different times after iron treatment, revealed ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves, reaching a maximum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

키워드

alignment amino acid sequence cDNA library Fpl gene iron storage protein Northern blot Southern blot.