Effect of Plant Growth Regulators on Plant Regeneration and in vitro Flowering Through Somatic Embryogenesis of Gentiana scabra

• 발행기관: 한국자원식물학회
• 저자명: Kim Young-Sook Choi Chang-Hak Kim Hyun-Soon Ko Jeong-Ae
• 간행물 정보: 『Plant Resources』Vol.8 No.2, 81~86쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2005.08.31

영문 초록

An efficient plant regeneration system of Gentiana scabra through somatic embryogenesis was established. Leaves and roots of seedlings of Gentiana scabra excised after germination were cultured on MS basal medium with 2,4-D, NAA or BA. Embryogenic callus was obtained on MS medium with 0.5 mg/L 2,4-D alone or 0.1 mg/L 2,4-D combimation with 1.0 mg/L BA after 45 days of culture. These embryogenic calli gave rise to somatic embryos, which subsequently developed into plantlets on MS medium without PGRs. Also, shoots were effectively differentiated from embryogenic callus when root segments were cultured on MS medium supplement with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Shoots were effectively rooted on MS medium without PGRs. In vitro flowers were formed from plantlets cultured on MS medium with 5% sucrose after 60 days of culture.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

LITERATURE CITED

키워드

In vitro flowering plant growth regulators somatic embryogenesis