오미자 잎 분획물의 B16F10 멜라노마 세포 내 색소침착 억제 효과

• 영문명: Inhibitory Effects of Pigmentation in B16F10 Melanoma Cells from Schizandra chinensis Fractions
• 발행기관: 한국인체미용예술학회
• 저자명: 김한혁(Han-Hyuk Kim) 나형진(Hyung-Jin Na) 정아름(A-Leum Jeong) 서미자(Mi-Ja Seo)
• 간행물 정보: 『한국인체미용예술학회지』한국인체미용예술학회지 제21권 제1호, 171~182쪽, 전체 12쪽
• 주제분류: 예술체육 > 예술일반
• 파일형태: PDF
• 발행일자: 2020.03.20

영문 초록

The Melanin synthesis is controlled by a series of enzymatic reactions regulated at the level of tyrosinase in melanocytes and melanoma cells. In an ongoing search for new tyrosinase inhibitors from natural sources, the dried leaves of Schisandra chinensis were extracted with 70% acetone and separated into fractions 1-3 (Fr.1-Fr.3). All fractions were evaluated for their inhibitory activity potency against the tyrosinase, and Fr.2 showed potent tyrosinase activities with 34.7% inhibition concentration 1,000 μg/mL compared to the positive control, albutin (23.5%). Finally, melanin contents was measured to determine the whitening effect. Fr.2 was inhibited at 19.3% at 50μg/mL, respectively. We investigated the changes in protein level of tyrosinase, Melanocyte Inducing Transcription Factor (MITF) by using western blotting. Fr.2 was able to significantly inhibit tyrosinase expression in protein level. These results suggest that Fr.2 inhibited melanin production which may be dependent on tyrosinase activity and expression in B16F10 melanoma cells, and an effective whitening agent for the skin.

목차

Ⅰ. 서론

Ⅱ. 재료 및 방법

Ⅲ. 결과 및 고찰

참고문헌

키워드

오미자나무 색소침착 멜라닌 생합성 티로시나제 B16F10 멜라노마 세포 Schisandra Chinensis Pigmentation Melanin Synthesis Tyrosinase B16F10 Melanoma Cells