국내 허가된 유전자재조합의약품 품질시험에 사용되는 전하변이체 분석법 조사연구

• 영문명: A Comparison Study on Charge Variant Assays of Recombinant DNA-derived Biologics Approved in Korea
• 발행기관: 대한약학회
• 저자명: 엄준호(Joon Ho Eom) 백정희(Jounghee Baek) 홍영기(Young Ki Hong) 손애라(Aera Son) 강소영(Soyeong Kang) 오호경(Hokyung Oh) 김민정(Min-Jung Kim) 우정남(Jeong-Nam Woo) 안치영(Chiyoung Ahn)
• 간행물 정보: 『약학회지』제64권 제1호(2020년), 73~78쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2020.02.29

영문 초록

Analysis on approval documents of recombinant DNA-derived biopharmaceuticals (172 items) marketed in Korea showed as followed. First of all, antibody drugs are about 37% among the biopharmaceuticals and most of antibodies as a major ingredient (about 92%) are humanized or human type. Second, charge variant assays using in quality (purity) test are distinctly different between antibody drugs and non-antibody drugs. The former prefer CEX and cIEF, the latter IEF and CZE. The preference difference may be related to size and complexity of target molecules. Third, the result of analysis on usage of 4 assays (CEX, CZE, IEF, and cIEF) in accordance with approval period indicated that cIEF usage got increased since 2010 and was about 50% after 2015. And finally, acceptance criteria of charge variant assays showed a few distinguished patterns independent of drug products. In the light of these facts, whether or not to establish a charge variant method and acceptance criteria as a quality test, and which method to use may depend on the characteristics of the product and analysis conditions of other manufacturers. In particular, in biopharmaceuticals that contain polymer proteins as the main component, such as antibodies, charge variant analysis is important, and the use of modern analytical methods such as the icIEF method is expected to increase.

목차

서 론(Introduction)

연구 방법(Research Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

감사의 말씀(Acknowledgment)

Conflict of Interest

References

키워드

Charge variant biopharmaceutical quality test purity