A SPORTS Nutrition Supplement, Albizia Julibrissin Extract Fermented with Kefir Yogut Regulates Lipopolysaccharideinduced Inflammatory Signal

• 발행기관: J-INSTITUTE
• 저자명: Kang Hye-ra Yang Han-joon
• 간행물 정보: 『Kinesiology』Kinesiology vol.4 no.2, 1~6쪽, 전체 6쪽
• 주제분류: 예술체육 > 체육
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Although the inflammatory response is vital for biological regulation, chronic inflammation damages the hu-man body. Presently available anti-inflammatory drugs, including steroids, have side effects that prevent their administration to athletes. This study aimed at elucidating a method to control exercise-induced inflammation and oxidative stress, by investigating the anti-inflammatory and antioxidant efficacy of extracts of Albizia juli-brissin fermented with kefir yogurt(FKA) in vitro. We investigated the effect of FKA on RAW 264.7 cells using cell viability assays. The regulatory effect of FKA in lipopolysaccharide(LPS)-induced nitric oxide(NO) production was determined using Griess reagent and the reverse transcription polymerase chain reaction(RT-PCR) assays, with specific primers such as interleukin(IL)-1β, IL-6, and IL-10, inducible nitric oxide synthase(iNOS), and cyclooxygen-ase- 2(COX-2) in vivo. Furthermore, the 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging assay was per-formed and the cellular morphology changes were observed using florescence microscopy. The results showed that FKA significantly reduced LPS–induced NO production by regulating the expression of IL-1β, iNOS, and COX-2; thus, FKA exerts cytoprotective effects against ROS-induced cell death. These data suggest the anti-inflamma-tory therapeutic potential of FKA.

목차

1. This Regulation is Aimed at the Sys-temization

2. Methods

3. Results

4. Discussion and Conclusion

5. References

키워드

Albizia Julibrissin Kefir Anti-Inflammation IL-1β iNOS