청국장에서 분리한 바실러스 종으로부터 베타-글루코시다제의 분석 및 부분정제

• 영문명: Identification and Partial Purification of the Extracellular p-Glucosidase of B a c illu s Species Isolated from Cheonggukjang, Fermented Bean Paste
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 문무상(Moo Sang Moon)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제19권 3호, 103~111쪽, 전체 9쪽
• 주제분류: 공학 > 산업공학
• 파일형태: PDF
• 발행일자: 2014.09.30

영문 초록

The strain obtained from Cheonggukjang produced a protein showing p-glucosidase activity in the experimental data of column chromatography and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. An extracellular p-glucosidase of B acillus species isolated from Cheonggukjang was identified and partially purified through 75% ammonium sulfate precipitation, High Q anion exchange chromatography, and Bio-Gel P-30 and P-60 size exclusion chromatography. The molecular weight of 45,000 dalton was estimated by SDS-PAGE. {3-Glucosidase was produced in the culture medium containing cellobiose, but not produced in glucose medium which may act as a suppressor for the enzyme expression. Assay of |3-glucosidase activity was performed by measuring the release of p-nitrophenol from the substrate p-nitrophenyl-|3-D-glucopyranoside.

목차

ᅵ . 서 론

II. 실험재료 및 방법

III. 실험결과

IV. 고 찰

V. 결 론

참고문헌

키워드

Bacillus(바실러스) |3-Glucosidase(베타-글루코시다제) Purification(정제) Chromatography(크로마토그래 피)