분광광도법에 의한 혈중일산화탄소의 측정에 관한 연구

• 영문명: A Study on the Determination of Carbon monoxide in the Blood by Spectrophotometry
• 발행기관: 한국환경보건학회
• 저자명: 정근호(Keun Ho Chung)
• 간행물 정보: 『한국환경보건학회지』제2권 제1호, 49~51쪽, 전체 3쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1975.02.28

영문 초록

The accidents, homicides and suicides of carbon monoxide poisoning to increase in number every year are required precize, accurate, and rapid method for the determination of carbon monoxide in the blood samples. Here is the basis of this method for the determination of percentage saturation of hemoglobin by carbon monoxide which have found out to be suitable in laboratory as follows: A 0.1ml of blood is mixed with 20ml of 0.1% ammonium hydroxide, and 20mg sodium hydrosulfite is added to convert oxyhemoglobin to reduction hemoglobin. The absorbance is measured at 538nm and 578nm, the measurement was carried out within ten minutes of addition of sodium hydrosulfate.