RAPD 分析에 의한 無窮花 品種間의 類緣關係 및 遺傳的 變異

• 영문명: Phylogenetic relationship and genetic variation among varieties of Hibiscus syriacus based on RAPD analysis
• 발행기관: 한국육종학회
• 저자명: Seon Ha Lee(李善河) Chi Hyun Kim(金致賢) Won Sup Song(宋源燮) Ill Sup Nou(盧一燮)
• 간행물 정보: 『한국육종학회지』Vol.28 No.4, 445~456쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1996.12.30

영문 초록

Genetic variation and phylogenetic relationship among 84 varieties of Hibiscus syriacus were investigated by RAPD analysis. When total proteins of 16 varieties were analyzed by SDS-PAGE, many protein bands were detected, but no differences among the varieties were observed. The optimum condition for PCR reaction was 150 mM dNTP, 0.2 unit Tag polymerase, and 1.5 or 2.0 mM MgCl₂ for 10㎕ reaction volume. Amplication with twenty two random primers resulted in polymorphism among the varieties and therefore enabled us to study genetic variation study. A total of 121 bands were observed by the RAPD analysis of the 84 varieties, 2 to 8 bands for each primer. The number of average bands were 5.5 for all the primers used, and 5.81 and 4.67 for 16 OPB primers and for 6 TAA primers, respectively. No differences between 10-mer and 12-mer primers were observed in detected band numbers. Among the 84 varieties, the most closely related two varieties appeared to be Serenade and Shihai (similarity value=0.91), and the least closely related were Chungmu and Kijibato (0.39). The 84 varieties could be classified into 6 groups (group A-F) based on RAPD analysis. Generally, domestic varieties did not-fit into a single specific group, but belonged to groups A, B, D and E along with introduced foreign varieties, indicating most of the domestic varieties were developed by crossing with introduced foreign varieties. When 12 varieties with similar genetic backgrounds were further analyzed, a total of 99 bands were detected and the varieties could be classified into 3 groups at the similarity value of 0.5. Among Tanshim (2n), Paektanshim (2n) and Kaeryang-tanshim (4n), Tanshim and Kaeryang-tanshim were more closely related than Tanshim and Paektanshim. Shihai, a variety from Japan, showed closer relationship with Parangsae than with Chabae. These reasons were widely discussed in this paper.

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키워드

RAPD DNA marker Polymorphism Phylogenetic relationship Hibiscus syriacus