구기자나무 體細胞胚 人工種子 生産과 發芽

• 영문명: Artificial seed production from somatic embryos and its germination in Lycium chinense Mill
• 발행기관: 한국육종학회
• 저자명: Jae Ki Kim(金宰琪) Byung Weon Kim(金柄源) Kwang Soo Roh(盧光洙)
• 간행물 정보: 『한국육종학회지』Vol.28 No.3, 255~261쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1996.09.30

영문 초록

An artificial seeds was developed using somatic embryos derived from the leaf callus of Lycium chinenes Mill. Somatic embryos were singly alginate-encapsulated to be used as artificial seeds. When cultured on MS basal medium, 95% of the non-encapsulated and 49% of the encapsulated somatic embryos were capable of germination. Among various concentrations of sodium alginate tested, the best result for bead formation was obtained in 3% sodium alginate. Of all tested media, 52% of somatic embryos were germinated on 2 MS medium but up to 60% on 3/2 MS medium. Characoal supplemented to the sodium alginated solution promoted shooting and rooting. The highest germination(80%) was obtained when artificial seeds was encapsulated with sodium alginated containing 0.5% charcoal. Artificial seeds did not lose the capacity for germination even after storing at 4℃ for 45 days. Shoots and roots of the encapsulated somatic embryos were induced after 4 weeks on 3/2 MS medium without growth regulators. Whole plantlets were successfully transplanted to potting soil for futher growth.

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ABSTRACT

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摘要

인용문헌

키워드

Artificial seeds Somatic embryo Lycium chinenes Mill. Encapulation Germination