RAPD를 이용한 보리×밀 속간교잡종의 보리유전자 도입 확인

• 영문명: Detection of barley genes in barley×wheat intergeneric hybrids using random amplified polymorphic DNA(RAPD)
• 발행기관: 한국육종학회
• 저자명: 임용우(Yong Woo Rim) 홍병희(Byung Hee Hong) 남중현(Jung Hyun Nam) 박문웅(Moon Woong Park) 하용웅(Yong Woong Ha) 박광근(Kwang Geun Park) 신정섭(Jeong Sheop Shin)
• 간행물 정보: 『한국육종학회지』Vol.27 No.4, 417~422쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1995.12.30

영문 초록

Random amplified polymorphic DNA(RAPD) was employed as a genetic marker in order to verify the genetic introgression of barley(cv. Manker 16) to barley × wheat backcross(BC₂F₁) lines. In RAPD analysis of 12 random primers tested, four of the 14 progenies obtained from barley × wheat backcrosses expressed band unique only to barley using primer number 225 (5’-CGA CTC ACA G-3’), and all progenies except H6-2 and H6-3 expressed bands unique only to barley using primer number 274(5’-GTT CCC GAG T-3’). All progenies of the BC₂F₁ lines appeared to have at least one of barley polymorphic patterns through RAPD analysis. Somatic chromosome numbers of BC₂F₁ lines ranged between 46 to 49 and appeared to have 4 to 7 chromosomes of barley. Use of RAPD as a genetic marker for the detection of genetic introgression of barley in barley × wheat intergeneric hybrid derivatives was effective and useful.

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키워드

RAPDs Chromosome Polymorphism. Primer Intergeneric hybridization Barley (Hordeum vulgare L.) Wheat (Triticum aestivum L.)