In vitro plant regeneration from flower buds of populus deltoides and P. maximowiczii

• 발행기관: 한국육종학회
• 저자명: 정경호(Kyung Ho Chung) 김주학(Joo Hag Kim) 전영우(Young Woo Chun)
• 간행물 정보: 『한국육종학회지』Vol.25 No.3, 191~200쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1993.09.30

영문 초록

Small immature flower buds of Populus deltoides and P.maximowiczii were cultured on the MS medium containing 0.5㎎/ℓ BA and 0.5-1.0㎎/ℓ NAA for development and growth. Small slices of catkin 5-6㎜ in thickness prepared from the well-developed flower bud 3-4㎝ in length were culture on the MS medium containing 1.0㎎/ℓ BA+0.05㎎/ℓ NAA and 0.5㎎/ℓ BA+0.05㎎/ℓ NAA for adventitious shoot regeneration from P.deltoides and P.maximowiczii, respectively. Average 3.8 shoots from P.deltoedes and 4.1 from P.maximowiczii were regenerated from a slice of catkin. More than 20 shoots can be regenerated from a single mature catkin of poplar such as P.deltoides that is recalcitrant to shoot regeneration. Adventitious shoots were initiated from three different places of an explant, the outer and the cut surface of stalks, and the marginal part of small bracts. The development of immature flower buds in vitro was affected by collecting time. Those collecting in late March were not developed well into mature flower buds. among five different collecting times, July, Sep., Nov., Jan., and Mar.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSIONS

摘要

LITERATURE CITED

키워드

Plant regeneration Flower bud culture Poplar Populus deltoides Populus maximowicaii