單位生殖에 의한 감자 半數體植物 誘起 Ⅲ. 色素遺傳子 發現樣相에 따른 半數體植物 選拔

• 영문명: Induction of haploids through parthenogenesis in potato Ⅲ. Selection of haploids by pigment markers on seed and plant
• 발행기관: 한국육종학회
• 저자명: Hyun Mook Cho(趙賢默) Hei Young Kim Lee(金惠英) Il Gin Mok(睦一振)
• 간행물 정보: 『한국육종학회지』Vol.25 No.1, 1~11쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1993.03.30

영문 초록

Interspecific and Interploidal crosses were made between tetraploids and diploids to induce potato haploids. Tetraploids female parents were grouped into three different types according to the pigments on the stem. Pigments on seeds, seedlings and mature plants were used as phenotyphic markers to select haploids. Haploids were derived only from non-embryo spot seeds from all of the three types of the female parents. The positive selection for non-pigmented plants at seed and seedling stage was highly accurate to select haploid in the progenies of the female parents with green stem (group G), except the cv. Russet Burbank. Most of seedlings with white hypocotyl in the progenies of female parents with basal purple(group BP) or whole purple stem(group P), were haploids. However, 45.5% and 69.5% of the seedlings with white-purple hypocotyl derived from these groups were haploids, respectively. Because of the matroclinous effect of their female parents on plant pigmentation, the negative selection for purple pigmented plants at seed, seedling, growth and subsequently, harvest stage, was effective. As a consequence, a two step selection method was applicable for group BP and P ; select the seedlings with only white hypocotyl at seedling stage as haploids, and discard all the plants with purple pigments on the hypocotyl, stem and tuber skin according to gradual plant growth. Up to now, from 1,253 of non-embryo spot seeds, 199 haploid plants were confirmed by the phenotypic markers and subsequently root-tip chromosome counting. The efficiency of haploid induction through interspecific and interploidal crosses was affected by different tetraploids female parents.

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키워드

Interspecific and interploidal crosses Marker gene Ploidy level