밀과 보리 原形質體의 融合 硏究

• 영문명: Studies on the isolation and fusion of protoplasts from triticum aestivum L. and hordeae bulbosum L.
• 발행기관: 한국육종학회
• 저자명: Heung Bae Kim(金興培) Jai Heon Lee(李在憲)
• 간행물 정보: 『한국육종학회지』Vol.19 No.1, 64~69쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1987.03.30

영문 초록

Factors affecting isolation and fusion of mesophyll tissue from T. aestivum and H. bulbosum for producing somatic cell hybrids were studied. In case of both wheat and barley, the highest protoplast yield was obtained when tissues were submitted to Mannitol 0.7 M for 2 hours. High protoplast yield was resulted when a solution of Cellulase 3%, Macerozyme 0.8% and Pectolyase 0.03% was used for 4 hours. The favorable pH of Mannitol and enzyme solution was 5.8. The most effective concentration of PEG ranged from 30% to 40% for fusion of T. aestivum and H. bulbosum mesophyll protoplasts. The best density of protoplast was 2.5×10⁵/ml for cell fusion.

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