In vitro에 依한 2n과 4n의 × populus albaglandulosa의 葯培養에 關한 硏究 Ⅰ. Anther의 成熟度에 따른 callus形成

• 영문명: On the anther culture of 2n and 4n × populus albaglandulosa on in vitro Ⅰ. Callus induction in maturity of anthers
• 발행기관: 한국육종학회
• 저자명: Chung Suk Kim(金鼎錫) Joo Soo Choi(崔柱銖) Ki Sik Youn(尹基植)
• 간행물 정보: 『한국육종학회지』Vol.19 No.1, 1~7쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1987.03.30

영문 초록

This study was conducted to induce the pollen plants from anther culture of Populus alba x P.glandulosa. However, the immediate objective was to examine the callus formation for each of anther maturities. Callus formation and differentiation from anther being at early leptotene stage during the first division were the best at 15℃ under 100 luxes on W.S. medium containing 0.1ppm of 2,4-D, at 25℃ under 100 luxes on W.S. medium containing 0.1 to 0.7ppm of NAA and at 15℃ under 100 luxes on W.S. medium containing 1.0ppm of BAP. Those at pachytene stage were the best at 25℃ under 100 luxes on W.S. medium containing 0.7ppm of 2,4-D and at 29℃ in the darkness on W.S. medium containing 0.06 to 0.15ppm of BAP. And those, however, at metaphase and anaphase were the best at 25℃ in the darkness on W.S. medium containing 0.lppm of 2,4-D and at 29℃ in the darkness on W.S. medium containing 0.06ppm of BAP. Among the above three stages, the early leptotene stage was the most effective on callus formation and differentiation. Callus formation at the pachytene stage was the best on W.S. medium containing various hormones while its differentiation was the best on W.S. medium containing 1.5ppm of 2,4-D. Callus differentiation of the 2n Populus hybrid grown on W.S. medium containing 1ppm of 2,4-D and 1 ppm of NAA was better than that of 4n Populus hybrid. Quantitative differentiation of 2n anther callus on the medium of W.S. containing mixed by 1 ppm of 2, 4-D and 1 ppm of NAA was better then anther callus.

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