감자와 가지의 原形質體 融合

• 영문명: Protoplast fusion between patato and egg plants
• 발행기관: 한국육종학회
• 저자명: Young am Chae(蔡永岩) Kyu whan Choi(崔奎煥)
• 간행물 정보: 『한국육종학회지』Vol.18 No.4, 10~13쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1986.12.30

영문 초록

This study was carried out to find out the proper enzyme mix for protoplast isolation, and the effects of PEG, pH and Ca ion on the fusion. Mix of 2% cellulase R10 with 0.5% macerozyme R10 and 2.5% cellulase with 0.75% macerozyme were proper for isolation of protoplasts from mesophyll tissues of potato and egg plant, respectively. Fusion frequency was higher in each of 35% PEG(MW 6000), 15 mM Ca ion and at pH 5.5, however, their combined effect was not greater than as it was expected.

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