Random amplified polymorphic DNA와 fluorescent amplified fragment length polymorphism 분석을 통한 Salmonella spp.의 유전형 분석

• 영문명: Genotyping of Salmonella spp. by random amplified polymorphic DNA and fluorescent amplified fragment length polymorphism analysis
• 발행기관: 한국예방수의학회
• 저자명: 박신정(Sin Jeong Park) 이재일(Jae Il Lee)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.35, No.4, 220~230쪽, 전체 11쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.12.30

영문 초록

Random amplified polymorphic DNA (RAPD) and fluorescent amplified fragment length polymorphism (FAFLP) analyses were executed on a total of 28 Salmonella spp., including 6 ATCC reference strains, 2 isolates from outbreaks of food poisoning in Gwangju, and 20 isolates from carcasses. For RAPD analysis, four primers, named P1254, 23L, OPA-4, OPB-17 were used producing amplification fragments ranged from 0.18kb to 2.6kb. As a result, 5 types using P1254, 5 types using 23L, 3 types using OPA-4, and 6 types using OPB-17 and a total of 18 RAPD types were achieved. For FAFLP analysis, bacterial genomic DNA was digested with endonucleases EcoRⅠ and MseⅠ, site-specific adaptors were ligated, and PCR amplification was carried out with an EcoR1 adaptor-specific primer labelled with fluorescent dye. Amplified fragments, which were separated on a polyacrylamide sequencing gel ranged from 35bp to 300bp were analysed. Results were displayed as a dendrogram with genetic distance. Twenty two Salmonella isolates and 6 reference strains were divided into 14 groups in a level of 0.136 genetic distance. In conclusion, Salmonella isolates of chicken carcasses have different genetic properties when compared to reference strains and isolates from outbreak of food poisoning.

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Salmonella spp RAPD FAFLP Food Poisoning