Determination of aminoglycosides in milk by liquid chromatography with tandem mass spectrometry

• 발행기관: 한국예방수의학회
• 저자명: Chae-Mi Lim Byung-Hoon Cho Gap-Su Chung Seong-Wan Son
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.36, No.3, 121~130쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.09.30

영문 초록

A confirmatory method based on liquid chromatography with tandem mass spectrometry was developed for deter-mination of 12 aminoglycosides in milk. Extraction of aminoglycosides from milk was performed using by liquid extrac-tion using a 10 mM phosphate buffer containing 2% (w/v) trichloroacetic acid, followed by performance of a solid-phase clean-up procedure on a hydrophilic-lipophilic balance solid-phase extraction (HLB SPE). Ion-pair chromatography, using a mixture of 20 mM heptafluorobutyric acid (HFBA) in water and acetonitrile as the mobile phase, was used for retention of aminoglycosides on a reversed-phase C 18 column. Mass spectral acquisition was performed in the multiple reaction monitoring mode, selecting two precursor ion>product ion transitions for each target compound. Satisfied recoveries (70.1～109.6%) of all aminoglycosides were demonstrated in spiked milk at three levels from 50 ng/g to 200 ng/g. The coefficients of variation ranged from 3.2% to 14.0%. The limits of quantitation (LOQs) for aminoglycosides ranged from 2.5 ng/g to 40.3 ng/g.

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키워드

Aminoglycosides LC-MS/MS Milk