Development and Evaluation of QTL-NILs for Grain Weight from an Interspecific Cross in Rice

• 발행기관: 한국육종학회
• 저자명: Yeo-Tae Yun Dong-Min Kim In-Kyu Park Chong-Tae Chung Yeaul-Kyu Seong Sang-Nag Ahn
• 간행물 정보: 『한국육종학회지』Vol.42 No.4, 357~364쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2010.08.30

영문 초록

In a previous study, we mapped 12 QTLs for 1,000 grain weight (TGW) in the 172 BC 2 F 2 lines derived from a cross between Oryza sativa ssp. Japonica cv. Hwaseongbyeo and O. rufipogon. These QTLs explained 5.4 – 11.4% of the phenotypic variance for TGW. Marker-aided selection combined with backcrosses was employed to develop QTL-NILs for each QTL. BC 2 F 2 lines with each target QTL were backcrossed to Hwaseongbyeo twice and then allowed to self to produce BC 4 F 5 populations. SSR markers linked to TGW were employed to select QTL-NILs with the respective target QTL. Six QTL-NILs with the recurrent parent, Hwaseongbyeo were evaluated for nine traits for three years from 2007 and 2009. Differences were observed between each of the 6 QTL-NILs and Hwaseongbyeo in TGW. In addition to TGW, these QTL-NILs displayed differences in other agronomic traits possibly indicating a tight linkage of genes controlling these traits. The direction of the QTL for TGW in 6 QTL-NILs was consistent as in the BC 2 F 2 lines from the same cross. Difference in TGW between each of the QTL-NILs and Hwaseongbyeo was associated with the difference in one or two grain shape traits; grain length, grain width, and grain thickness. SSR markers linked to the QTL for TGW will facilitate selection of the grain shape character in a breeding program to diversify grain shape and provide the foundation for map-based gene isolation. Also, the QTL-NILs developed in this report and the progenies from crosses between the QTL-NILs will be useful in clarifying epistatic interactions among QTLs for TGW.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

LITERATURE CITED

키워드

Rice QTL grain weight grain shape near isogenic line